Affinity Chromatography Scholarly Peer-review Journal

Affinity chromatography is a method of separation based on a specific binding interaction between a ligand being immobilized and its binding partner. Examples include antibody / antigen, enzyme / substrate, and enzyme / inhibitor interactions. Depending on the specificity of the interaction, the degree of purification can be quite high, and therefore it is generally the first step, if not the only step, in a purification strategy. This section provides an overview of affinity chromatography of proteins, general considerations for affinity purification of proteins, and provides some commonly used affinity chromatography methods. Scholarly peer review is the process of subjecting the scholarly work, research, or ideas of an author to the scrutiny of others who are experts in the same field before a paper is published in a journal describing this work. Work may be accepted, deemed acceptable with revisions or dismissed. Peer review requires a community of experts who are qualified and able to carry out reasonably impartial review in a given (narrowly defined) field.

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