ISSN: 1755-5191

Imaging in Medicine

Molecular Cloning Scholarly

 Molecular cloning one of the most fundamental procedures available for modern molecular biology research, has been critical for driving biotechnological advances. One of the main objectives in the post-genomics era is to functionally map gene expression data. Thus, developing methods for the rapid and efficient construction of various vectors for transgenic research is more critical now than ever before.     To the best of our knowledge, of the many molecular cloning protocols that have been developed, the following are the main techniques currently used for routine cloning: restriction digestion- and ligation-based cloning (Cohen et al., 1973), Gateway cloning (Hartley et al., 2000), Gibson assembly (Gibson et al., 2009), and Golden Gate cloning (Engler et al., 2008). Each of these methods have specific limitations (Liang et al., 2017). Traditionally, type II restriction endonucleases and DNA ligases have been used to construct recombinant plasmids, and these enzymes are still extensively used for diverse molecular biology applications. However, this method is laborious and time-consuming and is often limited by the relatively few available restriction enzyme sites, especially during the assembly of complex plasmids from multiple elements (Lampropoulos et al., 2013; Wang et al., 2014). Gateway cloning is a very popular site-specific recombination system that exploits the lambda phage integration and excision mechanism.

High Impact List of Articles

Relevant Topics in General Science