Review Article - International Journal of Clinical Rheumatology (2018) Volume 13, Issue 1

Peripheral blood biomarkers in systemic sclerosis -associated interstitial lung diseases

*Corresponding Author:
Juan Chen
Rheumatology Department
Xiamen University and Fujian Medical University of Affiliated First Hospital
Xiamen, China
E-mail: [email protected]

Abstract

Abstract: Interstitial lung disease (ILD) can lead to significant morbidity and excess mortality in systemic sclerosis (SSc) patients. The early identification of patients at risk of progressive ILD is the main challenges for treating this highly variable disease. Although high resolution CT (HRCT), lung function tests (PFTs), bronchial lavage fluid (BALF), lung biopsy can be used to detect SSc-ILD, it is necessary to find reliable predictors for the disease progression. Peripheral blood biomarkers offer the advantages of being readily obtained, non-invasive, and serially monitored. New insights during the past several years have underscored the epithelial and endothelial cell injury with inflammatory and immune activation impacting of clinically significant SSc-associated ILD, but the pathogenesis of SSc-ILD is unknown. Some serum biomarkers including SP-D, KL-6, IL-9, MMPs, MCP-1, Defensins, IL-8 and CCL18 have identified to be associated with SSc-ILD. Future more cohorts, well-designed and long-term studies are needed to find more prospective biomarkers that influence SSc-ILD disease progression.

Keywords

systemic sclerosis (SSc), interstitial lung disease (ILD), peripheral blood biomarkers

Introduction

Systemic sclerosis (SSc) is a disorder of the connective tissue characterized by fibrosis of the skin, vascular abnormalities, and presence of auto-antibodies [1]. It is characterized by excessive deposition of extracellular matrix. Therefore, there is significant heterogeneity in organ progression and prognosis.

Interstitial lung disease (ILD) is a heterogeneous group of parenchymal lung disorders that share common radiologic, pathologic, and clinical manifestations [2]. It is characterized by lung parenchyma damage, accompanied by inflammation and fibrosis, and fibrosis is often incurable. The fibrosing forms of ILD are often incurable, and are associated with significant morbidity and mortality [2].

SSc is often accompanied by ILD. The incidence of SSc-ILD in the relevant literatures ranges from 45% to 90% [2,3]. A recent European League Against Rheumatism Scleroderma Trials and Research analysis revealed in a cohort of 3,656 SSc patients that ILD was present in 53% of cases with diffuse cutaneous SSc and in 35% of cases with limited cutaneous SSc [4]. Pulmonary involvement is the leading cause of death in SSc patients due to pulmonary fibrosis or pulmonary hypertension. By current data, 34% of SSc deaths are due to SSc-ILD [5], while in developing countries the death rate is as high as 44.6%.

ILD can be a subclinical form (imaging or physiological abnormalities, but not accompanied by symptoms), chronic progression, or may occur in an explosive, life-threatening manner. The clinical course of ILD is divided into two subtypes: acute/ sub-acute interstitial pneumonia (A/SIP), also called rapidly progressive ILD (RP-ILD), and chronic IP (CIP). The pathophysiological classification includes usual interstitial pneumonia (UIP), nonspecific interstitial pneumonia (NSIP), organizing pneumonia (OP), diffuse alveolar damage (DAD), desquamative interstitial pneumonia, and lymphoid interstitial pneumonia [6].

NSIP is the more common subtype of inflammation in ILD. 77% of SSc-ILD are NSIP [7]. A large number of clinical and pathological studies have confirmed that high resolution CT (HRCT) pattern in patients are correlated with pathologic NSIP and pathologic UIP [8,9]. NSIP pattern is associated with better patient outcome than UIP pattern. It includes reticular, frosted glass shadows, hollow, thickened honeycomb lung nodules, emphysema, bronchial vascular bundles, bullae, traction bronchiectasis, cobblestone like appearance, bronchial tree, bronchiectasis and so on [10]. The most common manifestation of NSIP is lobular reticular formation in the pleural and basal regions [11,12]. UIP is mainly represented by grid or honeycomb shadow. Different patterns in HRCT can reflect NSIP and UIP [6]. The extent of ILD lesions can be graded according to HRCT [13]. At present, the commonly used methods for clinical detection of ILD are HRCT, pulmonary function tests (PFTs), bronchoalveolar lavage fluid (BALF), lung biopsy. HRCT has now become the most common and sensitive imaging method for diagnosing ILD as it offers the most detailed images of the lungs [14-16]. HRCT and PFTs are the most sensitive to change [17]. The specificity of PFTs is low [18]. Bronchoalveolar lavage fluid is a sensitive marker for the future development of SSc in patients with ILD, but it is invasive test. Although the specificity of lung biopsy is high, the clinical operation is difficult and the practicability is low. Therefore, the search for both economical and non-invasive methods of examination is particularly important. Biomarkers of serum are not only easy to obtain, noninvasive, but also can be monitored continuously [19], moreover, the early diagnosis of subclinical ILD in SSc is an effective and feasible way to identify SSc- ILD, which can improve the prognosis and mortality.

Though it is not clear about the pathogenesis of systemic sclerosis associated with interstitial lung diseases, serum biomarkers, genes and lifestyle are involved in SSc-ILD progression [20]. Unknown factors initiate immune dysfunction between epithelial and vascular endothelial cell, activate fibroblast function and extracellular matrix production. The combination of epithelial and endothelial cell injury as well as inflammatory and immune activation progress pulmonary fibrosis in SSc [19].

This paper focuses on peripheral blood biomarkers in SSc-ILD and other connective tissue disease (CTD) associated ILD.

KL-6

Krebs von den Lungen-6 (KL-6) is an important serum marker for ILD [21,22]. It is a high molecular weight, mucin like glycoprotein secreted by type-II alveolar pneumocytes and bronchial epithelial cells in response to cellular damage and regeneration in patients with ILD [21]. KL-6 is a mucin-associated glycoprotein, which may be a trigger for TGF-β signaling and fibrosis [23].

The level of KL-6 as a predictive factor could be used to identify the clinical development of ILD [24]. Hideaki et al. [25] retrospectively analyzed the medical records of 29 patients with SSc-ILD. Measurement of serum KL-6 levels, pulmonary function tests, and HRCT performed in parallel were reviewed. They found serum KL-6 correlated positively with diffusing capacity of the lung for carbon monoxide (DLCO)(% predicted) and disease extent on HRCT, and the changes in serum levels of KL-6 were significantly related to the changes in forced vital capacity (FVC) in SSc -associated ILD. Their study suggests KL-6 can be a useful monitoring tool of SSc-ILD activity.

Masataka et al. investigated the relationship between SSc-ILD and prognosis [26]. They chose fifty patients with early-stage SSc-ILD who had never received disease-modifying drugs and were either observed for ≥ 10 years or died from ILD-related causes, and the Cox proportional hazard model was used to identify initial factors that correlated with end stage lung disease (ESLD) development. They found elevated KL-6 at initial assessment was highly correlated with ESLD development and the initial KL-6 level correlated with the forced vital capacity decline rate. Baseline serum KL-6 is a biomarker potentially useful for predicting FVC decline.

The KL-6 in the peripheral serum was described earlier, and the relationship between KL-6 and the severity and the activity of SSc-ILD in bronchoalveolar lavage fluid was studied [27]. Fifteen patients with early SSc and 12 healthy controls were subjected to BALF. Serum KL-6 were increased in SSc patients and correlated with BALF concentration of eosinophils. Patients with more widespread ground glass opacities (GGO) on HRCT were characterized in BALF by a higher eosinophil count and in serum by higher KL-6. Therefore, KL-6 levels from BALF and serum being correlated to findings on HRCT suggest that KL-6 can be used as a useful biomarker of presence and extent of lung fibrosis in SSc patients.

KL-6 is also an important biomarker in other CTD-ILD. Higher levels of KL-6 was a diagnostic marker in 68 CTD-ILD group than in either 45 CTD without pulmonary involvement group or 45 healthy control group [28]. CTD-ILD included 12 Sjogren syndrome related interstitial lung disease (SS-ILD), 18 rheumatoid arthritis related interstitial lung disease (RA-ILD), 3 polymyositis/ dermatomyositis related interstitial lung disease (PM/DM-ILD). Interestingly, in other studies on PM/DM-ILD, the level of serum KL-6 was significantly higher [29-32]. KL-6 could be treated as a biomarker of disease activity and therapy efficacy of CTD-ILD.

SP-D

Surfactant, a lipoprotein complex, was originally described for its essential role in reducing surface tension at the air-liquid interface of the lung. However, it is now recognized as being a critical component in lung immune host defense. They include SP-B and SP-C and hydrophilic proteins SP-A and SP-D [33]. SP-D levels are more sensitive than SP-A in detecting ILD as defined by CT [19]. The sensitivities and specificities for detecting CT-positive ILD in 42 patients with SSc were 33% and 100% for SP-A and 77% and 83% for SP-D, respectively [34].

In a small but prospective study of 35 patients with SSc-ILD followed over 1-10 years, SP-D levels were seen to definitely increase over time in 9 out of the 10 patients with worsening ILD, as defined by changes in symptoms, lung function, and imaging, compared to mild increases in only 3 out of 25 patients with stable or improving SSc-ILD [35]. Therefore, SP-D is closely related to SSc-ILD.

A current study [25] retrospectively analyzed the medical records of 29 patients with SSc. Measurement of serum SP-D levels, pulmonary function tests, and HRCT performed in parallel were reviewed. Serum SP-D concentrations were also measured with commercially available ELISA kits. They considered a decline in FVC of >0.08 L/year on the ROC curve and found a higher serum level of SP-D was a significant predictor of potential FVC decline in patients with SSc -ILD. Serum levels of SP-D correlate with SSc- ILD activity.

In addition, Takahashi H et al. [34] found a less-invasive and lung-specific clinical biomarker. They measured serum levels of SP-D by sandwich enzyme-linked immunosorbent assays in 42 patients with progressive systemic sclerosis (PSS) to evaluate their significance in relation to the presence of ILD and to assess their diagnostic merits. They found the levels of SP-D in sera were significantly higher in the CT-positive ILD group than in the CT-negative ILD group. The research shows imaging and laboratory tests complement each other and support each other, suggesting serum SP-D is a clinically useful tool for detecting ILD complicated with PSS.

Similar results were in other CTD-ILD. SP-D levels were significantly increased in 113 RA-ILD, and strengthened the presence of clinically evident and subclinical RA-ILD on computed tomography scan in two independent RA cohorts and may facilitate identification of RA-ILD at an earlier stage, potentially leading to decreased morbidity and mortality [36]. Another study [37] found that the serum SP-D level in PM/DM patients with ILD was significantly higher than in those without ILD. The incidences of decreased vital capacity (%VC) and of decreased diffusing capacity of carbon monoxidase were also significantly greater in patients with an elevated SP-D level than in those with a normal level. SP-D was also significantly elevated in PM/DM patients with ILD compared with those in the patients without ILD [30].

IL-9

Interleukin 9 (IL-9) is a T cell-derived cytokine that was initially designated as a Th2 cytokine. Interleukin 9 targets cells of the lymphoid, myeloid, and mast cell lineages and likely contributes to the development of autoimmune diseases [38-41]. Yanaba K et al. [42] determined serum IL-9 levels and their clinical associations in patients with SSc. Serum IL-9 levels were examined by ELISA in 71 patients with SSc and the Mann-Whitney U test was used to compare IL-9 levels. Serum IL-9 level was increased in patients with SSc, and was associated with lower frequency and severity of pulmonary fibrosis in SSc. IL-9 could be a protective factor against the development of pulmonary fibrosis in this disease. They studied the relationship between the SSc and the clinic, but there was no convincing combination of imaging findings.

Other study [43] investigated the expression of IL-9 levels in SSc-ILD patients and the association of IL-9 with disease activity to better understand the relationship and immunopathological role of IL-9 in SSc-ILD. The peripheral blood samples were obtained from 9 patients with SSc. Determination of ILD was according to the HRCT performance, and the patients were divided into a SSc-ILD group and a simple SSc group according to presence or absence of ILD. They found serum IL-9 levels were significant higher in SSc- ILD and simple SSc patients than they were in healthy controls and that the levels were elevated in SSc-ILD patients compared with simple SSc patients. They also found the serum IL-9 levels were negatively correlated with FVC% and DLCO% in patients with SSc. Therefore, IL-9 levels were elevated not only in patients with simple SSc but also in those with SSc-ILD, demonstrating that IL-9 may contribute to the progression of interstitial lung injury in SSc and the IL-9 levels are gauges of the degree of damage to pulmonary function in patients with SSc-ILD. The sample size is too small to convince them of the experiment.

Serum IL-9 levels were also studied in other CTD-ILD. Serum IL-9 levels were significantly higher in 29 CTD-ILD and simple 32 CTD patients than 20 healthy controls. The IL-9 levels were elevated in CTD-ILD including 7 RA-ILD and 7 DM-ILD patients compared with simple CTD patients [43].

MMPs

The matrix metallo-proteinases (MMPs) are a family of zinc- and calcium-dependent endopeptidases that have the ability to degrade all major connective tissue matrices. The MMPs are involved in a variety of tissue remodeling under normal and pathological conditions. The MMPs can cleave most of the constituents of the ECM including collagen, proteoglycan, laminin, fibronectin and elastin [44]. The potent proteolytic activities of MMPs are mainly regulated by the balance with specific tissue inhibitors of Matrix metallo-proteinases (TIMP). Excessive or inappropriate expression of MMP may contribute to the pathogenesis of tissue destructive processes in a wide variety of diseases including lung diseases [45].

MMP-9, a type-IV collagenase, also called gelatinase B because of its ability to break down gelatin, has substrate specificity for a variety of ECM constituents such as native collagens IV and V, gelatin, elastin and vitronectin and has been observed to play an important role in lung tissue remodelling in an experimental model of lung fibrosis [44]. MMP-9 is produced constitutively by neutrophils and eosinophils, stored in granules and immediately released in the pro-form upon chemokine stimulation [46].

One study [47] emphasized that MMP-9 plays an important role in ECM refactoring of SSc- ILD by measuring levels of MMP-9 in BAL fluid from SSc patients with signs of ILD on HRCT, in SSc patients with normal HRCT and in healthy controls and related these levels to lung function. They chose nine SSc patients with ILD, seven SSc patients without ILD and 16 ages- and sex-matched healthy controls. Their experimental results were total MMP-9 and pro-MMP-9 levels were significantly increased in BAL fluid from the nine SSc patients with signs of ILD on HRCT compared to levels in healthy controls and compared to levels in SSc patients with normal HRCT. In SSc patients with ILD, calculated active MMP-9 levels were significantly higher than in SSc patients without ILD and tended to be higher than in healthy controls. And they proved the levels of total-MMP-9 and pro- MMP-9 were all negatively associated with TLC (total lung capacity), MMP-9 levels, regardless if expressed as total-, pro- or active MMP-9 were all inversely associated with TLC, consistent with a role for alveolar MMP- 9 in remodeling and lung volume reduction.

MMP-12 is a member of MMPs secreted extracellularly [48] and expressed in several cell types, and degraded several ECM proteins. Increasing evidence also indicates that MMP- 12 is involved in types of pathological lung tissue remodeling such as chronic pulmonary inflammation and fibrosis [49-51].

Serum MMP-12 levels were raised in SSc- ILD compared with patients without ILD by ELISA and correlated with severity of lung restriction from 72 SSc patients and 42 healthy controls. Immunohistochemical expression of MMP-12 were found in capillary vessels, inflammatory cells, alveolar macrophages and fibroblasts in the thickened alveolar septa in SSc-ILD, while faint expression in normal lung tissue, which were from lung biopsies of three SSc-ILD patients and five healthy controls. The conclusion is that MMP-12 levels are increased in SSc patients and involved in the pulmonary fibrosis of SSc-ILD [52].

Although there has been no literature on the relationship between MMP-7 and SSc-ILD, MMP-7 were identified by multiplex ELISA as potential biomarkers for RA-ILD in 133 Chinese RA cohort (50 RA-no ILD, 41 RA-ILD, 42 RA-indeterminate ILD) as well as in an independent cohort of US patients with RA and different stages of ILD (22 RA-no ILD, 49 RA-ILD, 15 RA-indeterminate ILD) [13]. Levels of MMP-7 were elevated in the serum of RA-ILD, supporting their value as pathogenically relevant biomarkers that can contribute to noninvasive detection of this extra-articular disease complication.

CXCL-10

CXC chemokines attract neutrophils and promote their adhesion to endothelial cells [53]. CXCL10 activate the function of Th1 lymphocytes secreted interferon (INF). CXCL10 levels are a reliable marker of aggressive Th1-mediated autoimmune disease [54]. The high serum levels of Th1 chemo-attractant CXCL10 and Th2 chemical attractants were found in only one cross-sectional study in SSc [55].

One study investigated serum levels of CXCL10 in ILD associated with SSc patients. CXCL10 level from patients with SSc was significantly higher than that from healthy volunteers, and the median CXCL10 level from SSc patients with ILD was significantly higher than that from SSc patients without ILD. CXCL10 is specifically increased in SSc patients with the lung involvement. CXCL10 plays a role in scleroderma lung disease. ILD were identified by CT in the study [56,57]. The untreated SSc patients with relatively short disease duration had high serum levels of CXCL10. Meanwhile high values of CXCL10 seemed to be associated with more severe clinical phenotype, namely with the presence of lung fibrosis. This provides a reference for the early diagnosis of SSc, especially SSc-ILD [57].

The similar results as MMP7 in the same study, serum CXCL-10 levels were elevated in RA-ILD comparing with RA no ILD [13].

MCP-1(CCL-2)

CCL2 is known as monocyte chemo-attractant protein-1 (MCP-1). MCP-1 is a member of the C-C chemokines [58]. In vivo studies suggest that MCP-1 recruits monocytes/ macrophages to sites of inflammation in a wide variety of pathological conditions, including ILD [59].

The plasma level of CCL2 is correlated with FVC value in SSc. However, there were no correlation between ILD severity and primary fibrotic genes such as collagen in the study [60]. That might be because skin fibrosis peaks early during the course of SSc and improves later [61], while fibrosis in pulmonary tissue continues to progress even at later stages of disease [62].

A study [63] examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution in the affected skin, of MCP-1. Elevated serum levels of MCP-1 significantly correlated with the presence of pulmonary fibrosis. MCP-1 was expressed in mononuclear cells or vascular endothelial cells in 41% (9/22) of SSc patients. The frequency of infiltrating mononuclear cells and endothelial cells that produced MCP-1 was significantly higher in SSc patients with early onset than in SSc patients with late onset. The abnormal accumulation of macrophages in the lungs may result from augmented local production of MCP-1 in the affected lungs of SSc, leading to elevated serum MCP-1 levels in patients with SSc. MCP-1 may play an important role in the development of pulmonary fibrosis in SSc.

Serum MCP-1 levels were significantly elevated in PM/DM patients with ILD compared with those in the patients without ILD in other study [30].

CCL18

The C-C motif chemokine ligand 18(CCL18) secreted from the alveolar macrophages can be activated by the Th2 cytokines, and increased in ILD such as SSc-ILD. Higher rates of disease progression occurred in higher serum CCL18 levels in a longitudinally over a 4-yr observation in SSc patients for the risk complication events which were defined as a 10% decrease from baseline of total lung capacity or forced vital capacity % predicted, or death, serum CCL18 levels. They found serum CCL18 levels are an accurate predictive biomarker for higher risk of subsequent scleroderma lung disease worsening [64].

Similarly, there is a study [65] assessed CCL18 in supernatants of cultured BAL cells as well as BAL fluid and serum samples from 43 patients with IIPs, 12 patients with SSc, and 23 healthy control subjects. CCL18 production by BAL cells and serum CCL18 concentrations reflect pulmonary fibrotic activity in patients with IIPs and those with SSc. Monitoring changes in CCL18 production might be an extraordinarily useful tool in clinical practice and in studies aimed at evaluating new approaches for treatment of fibrotic lung diseases.

Defensins and IL-8

The early studies found IL-8 were correlated with the respective BAL cell differentiation, lung function, and thoracic HRCT score in SSc patients. IL-8 levels were significantly increased in the ILD-positive SSc patients than ILD-negative, and negative correlations between the predicted DLCO levels and the BALF concentrations of IL-8. Predicted FVC values also correlated negatively with the BALF and IL-8 levels. IL-8 is the most-promising candidates for a targeted therapy in SSc-associated ILD [66].

Defensins are human neutrophil peptides (HNPs). It is a cationic antibacterial peptide rich in cysteine, which plays an important role in innate immunity and regulates the adaptive immunity of the host [67]. IL-8 is a potent neutrophil chemo-attractant [68]. Elevated plasma levels of HNPs were higher in acute exacerbation of interstitial pneumonia than in stable interstitial pneumonia, but not specific enough to serve as candidate biomarkers of acute exacerbation of interstitial pneumonia [66]. Recent study found BALF levels of HNPs and IL-8 were higher in SSc-ILD than in healthy controls, and are associated with various clinical disease parameters [68]. It is the first time to suggest that HNPs and IL-8 in BALF play a critical role in the pathogenesis of SSc-ILD [68]. HNPs induce the production of cytokines such as IL-8 and growth factors, which play pivotal roles in pulmonary fibrosis [66,69]. HNPs derived from neutrophils produce IL-8 in the lung. Defensins and IL-8 play a critical role in SSc-ILD [68]. This conclusion needs to be confirmed by increased patients number with no SSc-ILD controls and more cohorts with longitudinally study.

Kawasumi H [70] analyzed the associations between disease activity and levels of serum cytokines in PM/ DM. IL-8 were significantly correlated with rapidly progressive interstitial lung disease with PM/ DM, especially in the case of the RP-ILD subset than in the non- ILD subset or the chronic ILD subset.

Conclusion

Interstitial lung disease can lead to significant morbidity and excess mortality in systemic sclerosis patients. The early identification of patients at risk of progressive ILD is the main challenges for treating this highly variable disease. Although high resolution CT, lung function tests, bronchial lavage fluid, lung biopsy can be used to detect SSc- ILD, it is necessary to find reliable predictors for the disease progression. Peripheral blood biomarkers offer the advantages of being readily obtained, non-invasive, and serially monitored.

The epithelial and endothelial cell injury with inflammatory and immune activation impacting of clinically significant SSc-associated ILD, but the pathogenesis of SSc- ILD is unknown.

The improved biomarkers development in SSc- ILD including SP-D, KL-6, IL-9, MMPs, MCP-1, Defensins Defensins, IL-8 CXCL10 and CCL18. CCL18, SP-D and KL-6 are more readily available biomarkers in clinic for SSc-ILD. Future more cohorts, well-designed and long-term studies are needed to find more prospective biomarkers that influence CTDILD disease progression.

Disclosures

The authors have no financial relationships or conflicts of interest to disclose.

References

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