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 Next generation sequencing is a enormously similar or bottomless sequencing, in related terms that defines a DNA sequencing technology which has revolutionized genomic research and investigation. Using Next generation sequencing a whole human genome can be sequenced within a single day. In disparity, the preceding Sanger sequencing technology is also used to decode the human genome mandatory over a period to transport the final draft. Though in genome research Next generation sequencing has typically outdated conservative Sanger sequencing, it has not yet interpreted into monotonous clinical practice. The goal of this object is to evaluate the possible applications of Next generation sequencing in pediatrics.  

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