Annals of Clinical Trials and Vaccines Research

Mass Spectrometry Review Articles

Mass spectrometry (MS)-based proteomics measures peptides derived from proteins by proteolytic cleavage. Before performing the analysis by matrix-assisted laser desorption/ionization–tandem mass spectrometry (MALDI–MS/MS), nanoelectrospray–MS/MS (NanoES-MS/MS) or liquid chromatography–MS/MS (LC–MS/MS), the peptide mixtures need to be cleaned, concentrated and often selectively enriched or pre-fractionated, for which we employ simple, self-made and extremely economical stop-and-go-extraction tips (StageTips).Disks containing different surface functionalities and loose beads such as titania and zirconia for phosphopeptide enrichment can be combined.

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