ISSN: 1939-067X

Journal of Experimental Stroke & Translational Medicine

Protein Microarray

 Among all the proteomics technologies, protein microarray, over the last years, has gained an increased momentum within the biomarkers discovery domain. Protein microarray technology is an emerging field that provides a flexible platform for the characterization of  thousands of proteins in a highly parallel and high‐throughput manner. Protein microarrays are composed of two major classes. They are: analytical and functional. Additionally, tissue or cell lysates also can be fractionated and spotted on a slide to make a reverse‐phase protein microarray. Applications of protein microarrays, especially functional protein microarrays, had increased over the past decade because the fabrication technology has matured. Protein microarray is involved in deciphering cellular differentiation, transformation, angiogenesis, tumorigenesis, and metastasis. due to its development, it can detect alterations in protein expression levels, identify post-translational modification, mRNA events, and identify molecular networks triggered by therapeutic approaches, but also by environmental factors. The future of protein microarray is continuously expanding, so that profiling the cancer signaling network, personalizing therapy and improving diagnosis and prognosis would take a breakthrough. Protein microarrays were developed due to the restrictions of using DNA microarrays for determining gene expression levels in proteomics. the quantity of mRNA within the cell often doesn't reflect the expression levels of the proteins they correspond to.  

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