Protein Interaction Scholarly Open Access Journals
Protein–protein interaction prediction may be a field combining
bioinformatics and
structural biology in an effort to spot and catalog physical interactions between pairs or groups of proteins. Understanding protein–protein interactions is vital for the investigation of intracellular signaling pathways, modelling of protein complex structures and for gaining insights into various biochemical processes. Experimentally, physical interactions between pairs of proteins are often inferred from a spread of techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity purification/mass spectrometry, protein microarrays, fluorescence resonance energy transfer (FRET), and Microscale Thermophoresis (MST). Efforts to experimentally determine the interactome of various
species are ongoing. Experimentally determined interactions usually provide the idea for computational methods to predict interactions, e.g. using homologous protein sequences across species. However, there also are methods that predict interactions de novo, without prior knowledge of existing interactions. Proteins that interact are more likely to co-evolve, therefore, it's possible to form inferences about interactions between pairs of proteins supported their phylogenetic distances. it's also been observed in some cases that pairs of interacting proteins have fused orthologues in other organisms. additionally , variety of bound protein complexes are structurally solved and may be wont to identify the residues that mediate the interaction in order that similar motifs are often located in other organisms.
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