Cyclodextrin Glycosyltransferase Impact Factor
A CGTase isolated from Micrococcus varians produced CDs from starch in the presence of CaCl2 at 50–60°C and pH 6.0 during 2 days with trichloroethylene added to the medium, and the yield reached 60%. All CGTases can catalyze up to four reactions: cyclization, coupling and hydrolysis. All these activities share an equivalent catalytic mechanism which is common to all or any glycosyl-hydrolases.Cyclization is that the process by which a linear polysaccharid chain is cleaved and thus the two ends of the cleaved fragment are joined together to provide a circular dextrin (cyclodextrin or CD): on the idea of the amount of sugar residues this circular product is formed of three main sort of cyclodextrins are often distinguished, α-CD with six residues, β-CD with seven residues and γ-CD with eight residues.The coupling reaction is often easily described as the reverse cyclization process: the enzyme cleaves a cyclodextrin to supply a linear dextrin that is then joined to a linear oligosaccharide.Disproportionation is extremely almost like coupling, but the cleaved dextrin isn't a cyclodextrin, but a linear oligosaccharide that's then joined to a second oligosaccharide.CGTase also features a weak hydrolyzing activity in which the longer polysaccharide chains are cleared into shorter fragments.
High Impact List of Articles
Relevant Topics in General Science