Bioprocessing-innovations
Co-culture processes provide an ability to manufacture value-added goods from economic raw materials but a high-throughput
fermentation control technique is missing to study the temporal
physiology of
fermentation species in co-culture processes. For this research, we used shotgun proteomics to investigate a Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture cycle, and we tracked the
fermentation until glucose was depleted. Three time points were taken at 11.5 hours, 18.5 hours and 32 hours for proteomics research, which reflects the transition into diauxic change. We observed cellular dynamics within a 20-hour time span, using label-free quantitation. We distinguished the proteome from two yeasts, and from the most abundant S proteins. Stipitis, S. Cerevisiae contained expected glycolytic enzyme processes, histones, heat shock
proteins, ribosomal proteins and F1F0-ATPase proteins. We also established up-regulations for S after glucose depletion. Synthase stipitis malate and lyase isocitrate are the main enzymes in the glyoxylate cycle and gluconeogenesis. Gained expression of S. Diauxic change stipitis histone 2B was detected, and up-regulation of acetyl-CoA synthetase was indicated for histone acetylation. Without xylose, we observed NAD(P)H-dependent D-xylose reductase (Xyl1p) induction as early as 11.5 hours before glucose depletion. After glucose depletion we have detected the expression of D-xylulose reductase without xylose induction. Additional research is required to explore the cause of the xylose oxo-reductive pathway depression signals. The up-regulation of S, without induction of cellulose. Stipitis S indicated endo-1,4-beta-glucanase. Stipulates a policy to diversify carbon options following suppression of glucose. This research showed the application of shotgun proteomics in high-throughput monitoring of a complex co-culture system and in a position to elucidate the temporal
physiology of S. Stipitis.
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