An optotracer-based antibiotic susceptibility test targeting by biofilm

Author(s): Lucia Dong

A medical problem with worldwide implications is antimicrobial resistance. To prevent inefficient medication usage and the emergence of resistance, appropriate antimicrobial susceptibility testing is essential for drug development, patient diagnosis, and therapy [1]. Although bacterial biofilms play a significant role in persistent infections and illnesses brought on by medical devices, planktonic cultures are used to assess the effectiveness of medicines [2]. Here, we offer an optotracing-based biofilm AST using Salmonella as a model to address the demand for antibiotics that target bacteria living in biofilms [3]. The extracellular matrix components may be recorded in real-time using our non-disruptive approach, which allows for the precise identification of the biofilm lifestyle [4]. Thus, it is possible to confirm biofilm development before an antibiotic challenge and get pre-treatment information [5]. We performed a wide screen of the effects of antibiotics from various classes by introducing Kirby-Bauer discs [6]. Substances that have both stimulating and inhibiting effects on the ECM. Further research on these substances was conducted using agar-based dose-response biofilm-AST tests [7]. We obtained fresh data that directly reflected the treated biofilm by measuring the ECM based on the number of Curli and viewing the size and shape of the biofilm [8]. This demonstrated the effectiveness of numerous antibiotics in dismantling biofilms that had already formed, and it revealed fascinating potential resistance mechanisms that may have developed in response to therapies [9].