Annals of Clinical Trials and Vaccines Research

DNA,Nucleic ,molecular ,technology , Multi-gene,diagnostics,pharmacogenomics,analysis

As modern technology has been applied in medicine, scientists have always tried to understand the true nature of nucleic acids. To this end, the pioneering work of Sanger et al. in the late 1970s brought great innovation when they developed the most widely used method for sequencing DNA[1], still considered the gold standard in molecular diagnostics, although it is costly and time-consuming. Growing efforts have been made to broaden technical knowledge in this field until the discovery of the "second and third-generation sequencing" methodsThey are both part of next-generation sequencing ( NGS) technology, a group of techniques that revolutionize the standard concept of nucleic acids.NGS technology's great success is due to the ability to massively sequence millions of DNA reads, with the ability to perform multi-gene analysis, at least, using very low amounts of nucleic acids. NGS technology is also ideal for fast and effective decoding of complex genomes, with a consequent decrease in time and costs. In addition, it can also rely on a remarkable flexibility: in particular, its implementation has been recorded to have been effective in numerous research fields such as genetic disease molecular diagnostics, infectious diseases , cancer and pharmacogenomics

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