Next Generation Sequencing Seminars Open Access Journals
The massively parallel
sequencing technology referred to as next-generation
sequencing (NGS) has revolutionized the biological sciences. With its ultra-high throughput, speed and scalability next-generation
sequencing enables researchers to perform a good sort of applications and study biological systems at A level never before possible.
Today's complex genomic research questions demand a depth of data beyond the capacity of traditional DNA
sequencing technologies. Next-generation
sequencing has filled that gap and become an everyday research tool to deal with these questions.
Advantages of Next-generation
sequencing include Higher sensitivity to detect low-frequency variants. Faster turnaround time for high sample volumes. Comprehensive genomic coverage.
To perform Sanger Sequencing, you add your primers to a solution containing the genetic
information to be sequenced, then divide up the solution into four
PCR reactions. Each reaction contains a with dNTP mix with one among the four nucleotides substituted with a ddNTP (A, T, G, and C ddNTP groups).
The key difference between current next generation
sequencing techniques is the targeted enrichment step where
gene panels focus on a limited number of genes; whole exome
sequencing is focused on protein coding regions (~1−2% of the genome) and whole
genome sequencing does not require targeted enrichment.
Next generation methods of DNA
sequencing have three general steps: Library preparation: libraries are created using random fragmentation of DNA, followed by ligation with custom linkers. Amplification: the library is amplified using clonal amplification methods and PCR.
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