Cell Line High Impact Factor Journals
Cell culture and cell lines have assumed an important role in studying physiological, pathophysiological, and differentiation processes of specific cells. It allows the examination of stepwise alterations in the structure, biology, and genetic makeup of the cell under controlled environments. This is especially valuable for complex tissues, such as the pancreas, which is composed of various cell types, where in vivo examination of individual
cells is difficult, if not impossible. The extreme difficulties in the isolation and purification of individual epithelial
cells from complex
tissues by maintaining their native characteristics have hampered our understanding of their physiological, biological, growth, and differentiation characteristics.
Attempts have been made to culture almost every tissue, including neuronal cells, bone, cartilage, and hair cells. In general, animal cells, particularly fibroblasts, can be more successfully cultured than human cells, and human fibroblasts are easier to culture than epithelial cells. Also, different epithelial
cells show different responses to culture conditions. Despite advances in culturing techniques, human epithelial
cells could not be maintained in culture for long time periods. The problem is the tendency of human
cells to undergo senescence after a certain cell division. Transfection of these
cells with the E6E7 gene of human papilloma virus 16, or with the small and large T antigen of the simian virus 40, has partially overcome the senescence and has increased cell longevity in vitro but has not led to immortality of the cells. The resulting genetic manipulations limit the use of these
cells for molecular biological studies, especially for defining genetic changes that occur during cell differentiation and transformation.
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