Synthetic biology with metabolic engineering

Author(s): Emily George

The generation of microbial strains capable of manufacturing chemicals and materials effectively is made possible through metabolic engineering, but industrialising the strains takes a lot of time, money, and effort. Recently, high-performance strain generation has been aided by systems metabolic engineering, which combines classical metabolic engineering with systems biology, synthetic biology, and evolutionary engineering tools and tactics. This interdisciplinary approach has been continually enhanced over the last ten years in order to create overproducer strains that are competitive in the industrial market. This article reviews recent advancements in host strain selection, metabolic route reconstruction, tolerance augmentation, and metabolic flux optimization. It also reviews current trends in systems metabolic engineering, including tools and methodologies. Future difficulties and opportunities are also covered.