Polyphenolic Alkaline Magnetic Nanoparticles are Oxidizing Agents that Destroy Microbes

Author(s): Asfaq Faij

The function of the microbiome in chronic rhinosinusitis (CRS) is unknown. For acute disease exacerbations (AECRS), antibiotics are advised. Silver nanoparticles (AgNPs), an alternative treatment option, have been the focus of antibiotic resistance research. However, the Silver administration’s security is a concern. The biological activity of tannic acid-prepared AgNPs (TA-AgNPs) toward sinonasal pathogens and nasal epithelial cells (HNEpC) was the primary focus of this investigation. The well diffusion method was used to approximate the pathogens’ minimal inhibitory concentration (MIC) that were isolated from AECRS patients. A MTT assay and trypan blue exclusion were used to assess TAAgNPs’ cytotoxicity. Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiellaoxytoca, Acinetobacter baumannii, Serratia marcescens, and Enterobacter cloacae were among the 48 clinical isolates and four reference strains included in the study. The investigations revealed that the MIC values varied between segregates, even within species that are similar. After 24 hours of exposure, all of the isolates were sensitive to TA-AgNPs at concentrations that did not harm human cells. However, HNEpC’s therapeutic window was narrowed because 19% of pathogens were able to resist the biocidal action of the TA-AgNPs after 48 hours of exposure. TA-AgNPs were viewed as non-harmful to the analyzed eukaryotic cells and successful against most of microorganisms separated from patients with AECRS after brief openness; however, sensitivity testing may be required prior to application.