Cell cycle arrest and induction of apoptosis in human cancer cells lines by date palm kernels extracts and isolated cytotoxic compounds

Author(s): Mohamed E. S. Mirghani

This study conducted to identify the mechanism of cell death that induced by these isolated flavonoids, either by induction of apoptosis or necrosis. Three flavonoids namely nobiletin (NOB), tectorigenin (TEC) and persicognin (PERSI) were isolated from the optimised extractable polyphenols (EPP) crude extract of date palm kernels (DPK). The effect of crude EPP, NOB, TEC, PERS and the non-extractable polyphenols (NEPP) from DPK and paclitaxel on human lung cancer A549 and human colon cancer HT29 cell lines and on the normal murine fibroblast 3T3 cell line were investigated using MTT crystal violet and trypan blue exclusion assays. Apoptosis, one type of programmed cell death, is a well-defined self-suicide process counteracting tumor growth. Many chemotherapy drugs produce antitumor effects by triggering the apoptosis through a variety of molecular mechanisms. Apoptosis is either created by death receptors, which are called extrinsic pathway utilizing caspases 8 and 10. The other pathway is mitochondrial path or intrinsic pathway involving caspase. Recognizing involved mechanisms in cancer development is of great importance for developing neoplastic treatment.