Bio formulation for Cicer arietinum L's nutrient enhancement that includes cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8.

Author(s): Joel James

Ensifer adhaerens MSN12 and Bacillus cereus MEN8, two taxonomically distinct entophytic bacteria, form a group with promising plant growth promoting (PGP) properties. Through the creation of an efficient bio inoculant with cost-effective and readily available carriers, we demonstrate their transfer from the laboratory to the field. It was discovered that sugarcane straw ash (SCSA) is an effective carrier and bio formulation for enhancing strain viability and shelf life of up to one year. Seed germination, plant weight, length, seed yield, harvesting index, and proximate nutritional constituents were all increased by 20% over consortium treatment without SCSA in a bio formulation containing an SCSA-based consortium (MSN12 + MEN8). In addition, the bio formulation of post-harvest treated soil outperformed a pre-sowing SCSA-based bio formulation treated crop in terms of its physicochemical properties. The soil was enriched in a variety of proximate nutritional constituents, including dry matter (30 percent), crude protein (45 percent), crude fiber (35 percent), and ether extract (40 percent) in comparison to the control. A positive correlation between the treatments was found in scattered matrix plots and principal component analysis, confirming the T6 treatment’s improvement in soil nutrient components and proximate constituents (MSN12 + MEN8 + SCSA). For adequate delivery of laboratory strains as a substitute for chemi-fertilizers, the aforementioned results suggest that SCSA is effective not only as a carrier material but also as a support for microbial growth.