ISSN: 2041-6792

Clinical Investigation

Tomato Yellow Leaf Curl Virus

 "Tomato yellow leaf curl virus (TYLCV) genomic DNA molecule, a whitefly-transmitted geminivirus, was amplified by the use of loop-mediated isothermal amplification (LAMP) from total TYLCV-infected tomato extracts (Lycopersicon esculentum). The procedure was also used to amplify TYLCV DNA from individual whiteflies (Bemisia tabaci) total DNA extracts that had been fed on TYLCV-infected . One of the features of the LAMP method is its ability to synthesize an enormously large amount of DNA. Accordingly a large amount of by-product, pyrophosphate ion, is produced in the reaction mixture yielding a white precipitate of magnesium pyrophosphate."  

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