Annals of Clinical Trials and Vaccines Research

Southern Blot Open Access Journals

            Southern blot is a laboratory technique used to detect certain DNA sequences in blood or tissue samples. Restriction enzymes are used to cut DNA samples into fragments separated by gel electrophoresis. DNA fragments are transferred from the gel to the membrane surface. Southern Blot transfers can be used to clone based on homology based on the amino acid sequence of protein products from the target gene. Oligonucleotides are designed to be similar to the target sequence. If the DNA sequence is the basis or code for a protein molecule, certain interesting DNA molecules can be removed using the Southern blot technique. The amount of DNA required for this technique depends on the size and specific activity of the probe. Southern blot is a hybridization technique used to identify a number of DNA from a mixture of other similar molecules. This technique is based on the principle of splitting DNA fragments by gel electrophoresis and identified by hybridization labeled probes.  

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