ISSN: 2044-9038 (Print) 2044-9046 (Electronic)

Clinical Practice

Protein Sequencing Articles Top Open Access Journals

 Protein sequencing is that the sensible process of determining the amino acid sequence of all or a neighbourhood of a protein or peptide. This may serve to spot the protein or characterize its post-translational modifications. Protein sequencing is a highly sensitive technique that has been invaluable for providing critical primary structural data on isolated proteins, quality control for both recombinant proteins and synthetic peptides, and internal amino acid sequence from peptides derived from proteins that are used to design. Protein Sequence Analysis is that the method of subjecting a protein or peptide sequence to a minimum of one among an honest range of analytical methods to review its features, function, structure, or evolution. Methodologies used include sequence alignment, searches against biological databases, and other methods. Protein sequencing steps: 1. Splitting polypeptide chain: Protein moleculars should be separated and purified. Several polypeptides are combined together by non-covalent bond, which is understood as oligomeric protein. 2. Detecting the number of polypeptide in protein moleculars: The number of polypeptides can be determined by detecting the relationship between the number of moles of amino acid residues and protein molecular weight. 3. Breaking disulfide bonds: Several polypeptides chains are linked by disulfide bonds. Disulfide bonds will be reduced to thiol with excessive & [beta]- mercaptoethanol under the condition of 8mol / L urea or 6mol / L guanidine hydrochloride. And then it should be protected by alkyl reagents from re-oxidation.

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