ISSN: 2041-6792

Clinical Investigation

Pancreatic Islet Auto-transplantation Scholarly Open Access Journals

The pancreatectomy is performed in two stages: first, a distal pancreactomy (resecting the body and tail with or without the spleen), and second, a pancreaticoduodenectomy (resecting the head and uncinate process together with the duodenum). The procedure is most often performed by open laparotomy, although a robotic-assisted laparoscopic approach has been described.To minimize ischemia to the islet tissue, the gastroduodenal artery and the splenic artery and vein are preserved until just prior to resection. The spleen may be preserved on the short gastric vessels, although occasionally anatomic circumstances are favorable to enable pancreatectomy without sacrificing the splenic artery and vein. Pancreas processing After devascularization, the pancreas is promptly removed and placed in cold preservation solution. The duodenum and spleen are separated from the pancreas. The pancreatic duct is assessed for integrity, cannulated, and flushed with cold preservation solution, and blood is flushed from the major vessels. freeing islets from the exocrine tissue.At this point, the pellet containing acinar and endocrine tissue is collected . Direct infusion of large amounts of tissue into the portal vein leads to intrahepatic microembolization and inflammation .To prevent this, many islet centers further purify islets from acinar tissue when the pellet volume exceeds 0.25mL/kg body weight

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