Next Generation Sequencing Workshop Research Articles

The biological sciences were revolutionized by the massively parallel sequencing technology known as next generation sequencing (NGS). NGS enables researchers to conduct a wide range of applications and to study biological systems at a level never before possible with its ultra-high throughput, scalability, and speed. You add your primers to a solution containing the genetic information to be sequenced, and then divide the solution into four PCR reactions to perform Sanger sequencing. Each reaction contains a dNTP mix with one of the four nucleotides replaced with a ddNTP group (A, T, G , and C ddNTP). Next generation sequencing offers fast processing time and only takes about 4 hours to complete a cycle. NGS is good for: Cost-effectively testing > 100 genes at a time. Finding novel variants by increasing the number of sequenced targets in one round.