Short Notes on Meiotic and Mitotic Phenotypes Conferred by the blm1-1 Mutation in Saccharomyces cerevisiae

Author(s): Opeyemi Oluwasanmi Adeloye

Oxidative injury will cause variety of diseases, and may be fatal. The blm1-1 mutation of yeast confers hyper susceptibility to deadly effects of the oxidative, antineoplastic and antimitotic agent, bleomycin. For this report, additional defects given by the mutation in meiosis and cellular division were investigated. The viability of spores created throughout meiosis by homozygous traditional BLM1/BLM1, heterozygous BLM1/blm1-1, and homozygous mutant blm1-1/blm1-1 diploid strains was studied and compared. Close to half a mile of the tetrads derived from homozygous blm1-1/blm1-1 mutant diploid cells solely created one or two viable spores. In distinction, only 1 digit among all BLM1/ BLM1 and BLM1/blm1-1 tetrads solely created one or 2 viable spores. Rather, ninety four of BLM1/BLM1 tetrads and 100% of BLM1/blm1-1 tetrads created ace with four or 3 viable spores. Thus, a minimum of one copy of the BLM1 factor is crucial for the production of 4 viable spores once meiosis.