Disulfide-rich recombinant proteins from Plasmodium falciparum can be efficiently produced using Lactococcus lactis as a platform.

Author(s): David Gouveiac

Recently, it was discovered that Lactococcus lactis is an effective Gram-positive cell factory for the production of recombinant protein. This term “host” has been used by us and others to develop a few malaria vaccine candidates. Numerous clinical trials have confirmed this production system’s safety. In this study, we investigated L. lactis cell factories for the production of 31 representative Plasmodium falciparum antigens with varying sizes and predicted structural complexities. Among these antigens were eleven that had multiple predicted structural disulfide bonds, which are proteins that are thought to be difficult to produce.