Whole-genome Sequencing Scholarly Peer-review Journal:
Determining the order of DNA building blocks (nucleotides) in a person's ordering , called DNA sequencing, has advanced the study of
genetics and is one technique wont to test for genetic disorders. Two methods, whole exome
sequencing and whole
genome sequencing, are increasingly utilized in healthcare and research to spot genetic variations; both methods believe new technologies that allow rapid
sequencing of huge amounts of DNA. These approaches are referred to as next-generation
sequencing (or next-gen sequencing). The original
sequencing technology, called Sanger
sequencing (named after the scientist who developed it, Frederick Sanger), was a breakthrough that helped scientists determine the human ordering , but it's time-consuming and expensive. The Sanger method has been automated to form it faster and remains utilized in laboratories today to sequence short pieces of DNA, but it might take years to sequence all of an individual's DNA (known because the person's genome). Next-generation
sequencing has sped up the method (taking only days to weeks to sequence a person's genome) while reducing the value .With next-generation sequencing, it's now feasible to sequence large amounts of DNA, as an example all the pieces of a person's DNA that provide instructions for creating proteins. These pieces, called exons, are thought to form up 1 percent of an individual's genome. Together, all the exons during a
genome are referred to as the exome, and therefore the method of
sequencing them is understood as whole exome sequencing. This method allows variations within the protein-coding region of any
gene to be identified, instead of in just a get few genes. Because most known
mutations that cause disease occur in exons, whole exome
sequencing is assumed to be an efficient method to spot possible disease-causing mutations.
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