Whole-genome Sequencing Scholarly Peer-review Journal:

 Determining the order of DNA building blocks (nucleotides) in a person's ordering , called DNA sequencing, has advanced the study of genetics and is one technique wont to test for genetic disorders. Two methods, whole exome sequencing and whole genome sequencing, are increasingly utilized in healthcare and research to spot genetic variations; both methods believe new technologies that allow rapid sequencing of huge amounts of DNA. These approaches are referred to as next-generation sequencing (or next-gen sequencing). The original sequencing technology, called Sanger sequencing (named after the scientist who developed it, Frederick Sanger), was a breakthrough that helped scientists determine the human ordering , but it's time-consuming and expensive. The Sanger method has been automated to form it faster and remains utilized in laboratories today to sequence short pieces of DNA, but it might take years to sequence all of an individual's DNA (known because the person's genome). Next-generation sequencing has sped up the method (taking only days to weeks to sequence a person's genome) while reducing the value .With next-generation sequencing, it's now feasible to sequence large amounts of DNA, as an example all the pieces of a person's DNA that provide instructions for creating proteins. These pieces, called exons, are thought to form up 1 percent of an individual's genome. Together, all the exons during a genome are referred to as the exome, and therefore the method of sequencing them is understood as whole exome sequencing. This method allows variations within the protein-coding region of any gene to be identified, instead of in just a get few genes. Because most known mutations that cause disease occur in exons, whole exome sequencing is assumed to be an efficient method to spot possible disease-causing mutations.