Southern Rice Black-streaked Dwarf Virus
Their will increasing in growth at 20 days after the top of a 7-day exposure of virilifrrous S.furcifera Under a continuing temperature of 27°C, a considerable increase of SRBSDV titer within the leaf sheath alongside typical symptoms. Approximately 40% of S. furcifera acquired SRBSDV through feeding for five days on
rice plants that were infected following exposure to viruliferous vectors for 10 to fifteen days. These results suggest that
rice infected by S. furcifera are often a source of SRBSDV before subsequent generation of emerges. Infected plants showed stunting, dark leaf and little enations on stem and leaf back. The virus was transmitted to
rice seedlings by white-backed planthoppers, Sogatella furcifera (Hemiptera: Delphacidae), collected within the diseased fields. Analysis of dsRNA extracts from infected plants revealed ten linear segments, which were almost like the electrophoretic profile of
Rice black-streaked dwarf virus (RBSDV). RT-PCR with one primer which matched to a linker sequence ligated to both 3′ ends of the viral genomic dsRNAs resulted in amplification of
genome segments 9 (S9) and 10 (S10) cDNA products. These values were almost like those among other
viruses within the Fijivirus group 2 and considerably less than those among RBSDV isolates. It is proposed that this virus be considered as a replacement species, Southern
rice black-streaked dwarf virus, within the group 2 of the genus Fijivirus within the family Reoviridae.
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