ISSN: 2044-9038 (Print) 2044-9046 (Electronic)

Clinical Practice

Sequencing New Journal

 In genetics and biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched biopolymer. Sequencing employs a technique known as electrophoresis to separate pieces of DNA that differ in length by only one base. In electrophoresis, DNA to be sequenced is placed at one end of a gel—a slab of a gelatin-like substance. (A major part of DNA sequencing simply comes down to making a bunch of Jell-O.) 6.1 Long-read sequencing methods. 6.1.1 Single molecule real time (SMRT) sequencing. 6.1.2 Nanopore DNA sequencing. 6.2 Short-read sequencing methods. 6.2.1 Massively parallel signature sequencing (MPSS) 6.2.2 Polony sequencing. 6.2.3 454 pyrosequencing. 6.2.4 Illumina (Solexa) sequencing. The sequence tells scientists the kind of genetic information that is carried in a particular DNA segment. For example, scientists can use sequence information to determine which stretches of DNA contain genes and which stretches carry regulatory instructions, turning genes on or off. The massively parallel sequencing technology known as next-generation sequencing (NGS) has revolutionized the biological sciences. With its ultra-high throughput, scalability, and speed, NGS enables researchers to perform a wide variety of applications and study biological systems at a level never before possible.  

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