ISSN: 2044-9038 (Print) 2044-9046 (Electronic)

Clinical Practice

Comparitive-Genome-Hybridization Journals

Comparative genomic hybridization (CGH) is a molecular cytogenetic method for analyzing discrepancies in the number of copies (CNVs) relative to the degree of ploidy in the DNA of a research sample compared to a reference sample, without the need for cultivated cells. The purpose of this technique is to compare two genomic DNA samples easily and efficiently from two sources that are most often closely related because they are believed to contain differences in either the gains or losses of either whole chromosomes or subchromosomal regions.  This technique was originally designed to determine the variations between the chromosomal solid tumor complements and normal tissue. This is done by means of effective fluorescence hybridization in situ. In short, this involves isolating DNA from the two sources to be compared, most commonly a single stranded test and reference source, separate labeling of each DNA sample with fluorophores (fluorescent molecules) of different colors (usually red and green), denaturation of DNA, and hybridisation of the two resulting samples in a 1:1 ratio to a normal metaphase spring. Usage of a fluorescence and device microscope.    

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