Stem Cell Research and Regenerative Medicine

Cell Line Top Journals

Cell Culture and therefore the Establishment of Cell Lines Cell culture and cell lines have assumed a crucial role in studying physiological, pathophysiological, and differentiation processes of specific cells. It allows the examination of stepwise alterations within the structure, biology, and genetic makeup of the cell under controlled environments. This is often especially valuable for complex tissues, like the pancreas, which consists of varied cell types, where in vivo examination of individual cells is difficult, if not impossible. The acute difficulties within the isolation and purification of individual epithelial cells from complex tissues by maintaining their native characteristics have hampered our understanding of their physiological, biological, growth, and differentiation characteristics. Attempts are made to culture almost every tissue, including neuronal cells, bone, cartilage, and hair cells. Generally, animal cells, particularly fibroblasts, are often more successfully cultured than human cells, and human fibroblasts are easier to culture than epithelial cells. Also, different epithelial cells show different responses to culture conditions. Despite advances in culturing techniques, human epithelial cells couldn't be maintained in culture for while periods. The matter is that the tendency of human cells to undergo senescence after a particular cellular division. Transfection of those cells with the E6E7 gene of human papilloma virus 16, or with the tiny and enormous T antigen of the simian virus 40, has partially overcome the senescence and has increased cell longevity in vitro but has not led to immortality of the cells.    

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