Abstract

Liquid-liquid extraction method developed for thymoquinone from seed powder of Nigella sativa, characterized it by UVspectrophotometer

Author(s): Mohamad Taleuzzaman

Nigella Sativa Linn. (Ranunculaceae), commonly known as black seed or black cumin, is an herbaceous plant. Liquidliquid extraction method developed, the seed powder Nigella sativa was packed in a muslin cloth and placed in a beaker containing sufficient quantity of methanol for 72 hrs. Thereafter the methanolic extracts were filtered through Whatman filter paper no. 42 and the resultant filtrates were concentrated under reduced pressure using rotary evaporator characterize by UVspectrometer. The λmax of thymoquinone is found 254 nm and it confirms pure compound of thymoquinone.