ISSN: 2044-9038 (Print) 2044-9046 (Electronic)

Clinical Practice

Thin Layer Chromatography Research Articles

  Thin layer chromatography (TLC) remains a useful and cheap technique to screen organic chemical reactions, separate mixtures, and check purity and identity of compounds by way of a retardation factor (Rf). TLC has been employed for analysis of nucleotides, nucleosides, and nucleobases for quite half century. As for the quantification of 5-mC, genomic DNA is usually enzymatically hydrolyzed to nucleosides then specified with [E32P]ATP by T4 polynucleotide kinase followed by separation on TLC plates. TLC separation are often administered in either one or two dimensions, with the latter providing more resolution. The relative intensity of the spots are often determined Some new nucleotide adjustments, like 5-hmC and 5-caC were originally invented in genomic DNA from mammalian cells by TLC method then later confirmed by high-resolution mass spectrometry. Generally, TLC method is high-throughput, low cost and there's no need for classy instrumentation.TLC is that the conventional method for separating and enriching phytosterol classes. The vegetable oil is saponified with KOH and therefore the unsaponifiables are extracted with an appropriate solvent like hexane, ether, etc. The unsaponifiable components are then enforced to the TLC plate (silica gel).However, the TLC method has some drawbacks. Different sterol fractions have close Rf values in TLC, which can cause mixing during scraping of TLC bands. The method is also time-consuming and laborious. In addition, preparative TLC is disadvantageous because it's a coffee recovery rate.            

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