PCR Technology Peer-review Journals
PCR is shorthand for an easy but very useful procedure in biology called the polymerase chain reaction. It is a way wont to amplify a segment of DNA of interest or produce lots and much of copies. In other words,
PCR enables you to supply many copies of a selected DNA sequence from an initially small sample – sometimes even one copy. It is an important process for a variety of genetic technologies and, in fact, has enabled the event of a set of latest technologies.
PCR mimics what happens in
cells when DNA is copied (replicated) before cellular division , but it's administered in controlled conditions during a laboratory. The machine that's used is just called a
PCR machine or a thermocycler. Test tubes containing the DNA mixture of interest are put into the machine, and therefore the machine changes the temperature to suit each step of the method .
PCR technology is still developing. There is continuing development and refinement of the processes and tools used, allowing the method to be adapted to satisfy specialist needs. For instance, new methods and refinements are being developed and used, especially when quantification of DNA during a sample is required . New methods include real-time
PCR or quantitative
PCR (qPCR) and digital
PCR (dPCR). In qPCR, the amplification of DNA is monitored in real time, allowing the quantification of target DNA throughout the method . dPCR may be a new, more refined approach that breaks the
PCR process up into many smaller steps.
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