ISSN: 2041-6792

Clinical Investigation

Cell Culture Impact Factor

 Cell culture is the combination process by which the cells are enlarged under controlled conditions. A compatible presence of floating cells is a common phenomenon in cultures of human embryonic stem cells. It is presently believed that inevitable imperfections in culture conditions guide the cells to undergo senescence and apoptosis deriving in unattached cells floating in the culture medium. In human embryonic stem cell colonies, motivated by recent studies on mitotic activities, we believe the existence of floating cells is not simply the result of unfavorable growth conditions but an intrinsic phenomenon resulted from maintaining the pluripotency of hESCs under the culture conditions. Continuous cell cleaving beyond the colonies is responsible for the emergence of floating cells during hESC culture. This is utterly different from the bacterial colony growth where the cells in the center of colonies are quiescent.    

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