Cell Culture Impact Factor
Cell culture is the combination process by which the
cells are enlarged under controlled conditions. A compatible presence of floating
cells is a common phenomenon in cultures of human embryonic stem cells. It is presently believed that inevitable imperfections in culture conditions guide the
cells to undergo senescence and
apoptosis deriving in unattached
cells floating in the culture medium. In human embryonic
stem cell colonies, motivated by recent studies on mitotic activities, we believe the existence of floating
cells is not simply the result of unfavorable growth conditions but an intrinsic phenomenon resulted from maintaining the pluripotency of hESCs under the culture conditions. Continuous cell cleaving beyond the colonies is responsible for the emergence of floating
cells during hESC culture. This is utterly different from the bacterial colony growth where the
cells in the center of colonies are quiescent.
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