Abstract

Scheuermann's Disease can be Repopulated Using Blood from the Vertebra and Immature Stem Cells

Author(s): Hellen Marker

Stem Cells and peripheral blood are potential scaffolds and cell sources for Scheuermann’s Disease regeneration. The main purpose of this study is to investigate the effect of PRF scaffolds and autologous uncultured Stem Cells peripheral blood on regeneration of knee Scheuermann’s Disease in rabbits. Three different types of PRF scaffolds were generated from peripheral blood (Ch-PRF and L-PRF) and Stem Cells combined with uncultured peripheral Stem Cells(BMM-PRF). The histological properties of these scaffolds were evaluated using hematoxylin-eosin staining, picrosirius red staining, and immunohistochemical staining. Scheuermann’s disease defect 3 mm in diameter and 3 mm deep was created in the trochlear groove of the femur of a rabbit. Different PRF scaffolds were then used to treat the defect. A group of rabbits with an induced Scheuermann’s Disease defect and not treated with scaffolds was used as a control. Regeneration of osteochondritic tissue was determined macroscopically (internal cartilage repair association score, using X-ray) and microscopically (hematoxylin and eosin staining, safranin O staining, toluidine staining, and histological Wakiya scale, immunohistochemistry). In addition to gene expression analysis of Scheuermann’s Disease markers. Ch-PRF had heterogeneous fibrin network structure and cell populations. L-PRF and BMM-PRF had a homogeneous structure with a uniformly distributed fibrin network. Ch-PRF and L-PRF contained populations of CD45- positive leukocytes embedded in fibrin networks, whereas peripheral blood within the BMM-PRF scaffolds was sensitive to the pluripotent stem cell-specific antibody Oct-4. It was positive. Rabbits implanted with autografts showed significantly improved articular cartilage and subchondral bone healing compared to the untreated group. A gradual regeneration was observed after 2, 4, and 6 weeks of PRF scaffold treatment, which was particularly pronounced in the BMM-PRF group. A combination of biomaterials containing autologous platelet-rich fibrin and uncultured peripheral Stem Cellspromoted regeneration of Scheuermann’s Disease in a rabbit model more than platelet-rich fibrin material alone. Our results indicate that autologous platelet-rich fibrin scaffolds in combination with uncultured Stem Cellsand peripheral blood may be a suitable therapy in addition to stem cell and biomaterial therapy to heal osteochondritic lesions.